Hepatotoxicity Kit - Ready to Use

ACTIVTox accurately identifies liver specific toxins

The ACTIVTox cytotoxicity assay provides a quick, high through-put method for quantitatively measuring lactate dehydrogenase (LDH) release from lysed cells in a colorimetric, non-radioactive system. The ACTIVTox cell line provides the user with a platform that emulates an adult liver system complete with physiologically regulated drug disposal enzymes.

Kit Components

1. Cell Plate(s) with C3A cells
2. Cytotoxicity Assay Reagents
1. 10x Lysis Solution. Store at 2–8°C until needed for assay
2. Reconstituted Substrate Solution. Light sensitive. Store in a dark area until just before use. Store at –20°C until needed for assay
3. Stop solution. Store at 2–8°C until needed for assay.
3. Positive Control: 5mM Terfenadine (100x). Store at –20°C until needed for the assay.
4. Media #7 (MED #7). Store at 2–8°C until needed.
5. Protocol (pdf)

* Microplate Reader and ELISA Plate are not supplied. Suggested brand: Greiner No.655090