P450 CYP1A Induction Kit - Ready to Use

ACTIVTox responds accurately to CYP1A inducers

The ACTIVTox CYP1A assay is based on CYP1A, a member of the p450 enzymes in the liver. This enzyme can be induced by a variety of agents such as cigarette smoke, omeprazole, methylcholanthrene, and TCDD; it is inhibited by furafylline. One of the CYP1A substrates is ethoxyresorufin (ERes) and in the presence of CYP1A ERes is converted to the fluorescent compound, resorufin. The ACTIVTox CYP1A kit relies on this specific reaction to make ERes an excellent probe for CYP1A activity in the system.

Kit Components

1. Cell Plate(s) with C3A cells
2. CYP1A Assay Reagents
1. 1mM Ethoxyresorufin in DMSO (ERes). Store at 2–8°C until needed for assay.
3. Media #6 (MED #6). Store at 2–8°C until needed for assay.
4. Positive control: 500µM Methylcholanthrene (100x). Store at 2–8°C until needed for assay
5. Media #7 (MED #7). Store at 2–8°C until needed for assay.
6. Protocol (pdf)

* Fluorescence Plate Reader is not supplied.